Probing a rate-limiting step by mutational perturbation of AdoMet binding in the HhaI methyltransferase
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منابع مشابه
Probing a rate-limiting step by mutational perturbation of AdoMet binding in the HhaI methyltransferase
DNA methylation plays important roles via regulation of numerous cellular mechanisms in diverse organisms, including humans. The paradigm bacterial methyltransferase (MTase) HhaI (M.HhaI) catalyzes the transfer of a methyl group from the cofactor S-adenosyl-L-methionine (AdoMet) onto the target cytosine in DNA, yielding 5-methylcytosine and S-adenosyl-L-homocysteine (AdoHcy). The turnover rate ...
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The mechanism of DNA cytosine-5 methylation. Kinetic and mutational dissection of Hhai methyltransferase.
Kinetic and binding studies involving a model DNA cytosine-5-methyltransferase, M.HhaI, and a 37-mer DNA duplex containing a single hemimethylated target site were applied to characterize intermediates on the reaction pathway. Stopped-flow fluorescence studies reveal that cofactor S-adenosyl-l-methionine (AdoMet) and product S-adenosyl-l-homocysteine (AdoHcy) form similar rapidly reversible bin...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2005
ISSN: 1362-4962
DOI: 10.1093/nar/gki175